HBP1-mediated transcriptional repression of AFP inhibits hepatoma progression

Abstract Background Hepatoma is a common malignancy of the liver. The abnormal high expression alpha-fetoprotein (AFP) intimately associated with hepatoma progress, but mechanism transcriptional regulation and singularly activation AFP gene in not clear. Methods transcription factor HBP1 clinical significance were further analyzed tissues from patients who received surgery or TACE then monitored for relapse up 10 years. HBP1-mediated was by Western blotting, Luciferase assay, Realtime-PCR, ChIP EMSA. After verified axis HBP-AFP, its impact on measured MTT, Transwell FACS cells tumorigenesis ?/? mice. Results relative expressions correlated survival prognosis patients. repressed directly binding to promoter. Hepatitis B Virus (HBV)-encoded protein HBx promoted through directly. Icaritin, an active ingredient Chinese herb epimedium, inhibited enhancing transrepression AFP. repression attenuated effect PTEN, MMP9 caspase-3, thus proliferation migration, induced apoptosis cells. deregulation contributed progression Conclusions Our data clarify inhibiting suppression cells, providing more comprehensive theoretical basis potential solutions diagnosis treatment hepatoma.